HBICloud

Version: v1.0 Release Date: 2023.12.25 Last Update Date: 2023.12.25
Introduction: Used to design PCR primers to assist in DNA amplification, cloning, and sequencing in experiments.

Input: .csv, .xls, .xlsx, .xlsm

Sequence: ATCTTCTCATGCTTCTGTATGTGATTGTTTTTCTTCGCTCTTTTTTCCAGTCAATTTTGGTGCAATGCATATATTTGTCTTGAGAAATCGGGACTCCTTAAATCAATAGCCTTCTGTCAAAAGAGAGCTATCTTTTCACTTAGTGCTATGCCTAGCAAGCACTAGAGCTCCAATCATGCTACTCATAAAATAAGATTTGGAACCAAAAACCAGACGAAATAGTAGGTTTAAAGTAAATTGCTCAACGTTTCAAATTAGTGCAACTTTGCACAAACTGGCATAACCTGTATATTTCAGAGAGTTCTCTTTTCTTTGCATTGGTAGTAATGGAATGCTTTCATTGTCTAAAATGAAGAATATTTTACTATTTTGTAGTTGCACTTTTATCTATCATCTAAAAGTAACAATTCTGCTGCTTGATCATTTT

Output:

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Number To Return:

Primer Size(nt): Min Opt Max

Primer Tm(℃): Min Opt Max

Primer GC%: Min Max

Product Size:
Thermodynamic oligo alignment:
Max Poly-X: Internal Max Poly-X:
Salt monovalent: DNA conc:
Max self any: Max self end:
Pair Max compl any: Pair Max compl end:
GC clamp: Max NS accepted:

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Parameters

Result