We summarized the comparison and annotation of all small RNAs with other types of RNAs. Small RNA sequences are short, and it is generally easy to match different fragments, so there are multiple annotation results. In order to make each small RNA have a unique comment, the existing comment results are sorted according to the priority of known miRNA > rRNA > tRNA > snRNA > snoRNA > novel miRNA.
Materials and methods
First, the raw data is de-jointed and mass filtered, and the filtered sequences are de-processed (that is, the identical sequences are merged and the corresponding abundance of the sequences is recorded). Then, the de-duplicated sequences were compared with Rfan and miRBase databases to obtain the annotation information of various SRnas. If the species under test has a reference genome, the sequence to the reference genome will be compared for a new miRNA prediction analysis.
Statistical table of small RNA classification (Unique)
Sample
Known miRNA
Novel miRNA
rRNA
snoRNA
snRNA
tRNA
unknown
flower1
1071228
13081
3126020
20892
22497
300562
6266106
flower2
976862
14619
3591927
21000
23105
344321
6303216
flower3
1063654
14645
3319824
21647
23568
311196
6269926
fruit1
419238
4442
13755470
22118
51771
665068
5494100
fruit2
286935
2064
6303842
9444
20445
225574
2584993
fruit3
236114
1752
6512449
9816
20780
257915
2461577
leaf1
2590514
24213
2989495
18031
16659
441931
5338687
leaf2
2144999
21025
2933885
20392
15892
446749
5191911
leaf3
2104628
19357
2496628
16257
13855
383090
4385763
root1
434006
10590
3070174
15713
16171
677360
2782169
root2
442464
12281
3469433
17252
18015
697821
2986426
root3
1195523
31864
6775525
34530
47209
795681
8253047
seed1
34734
316
11211533
29034
100375
280954
2327555
seed2
39231
353
12258686
31937
111895
292857
2512325
seed3
33604
300
11857065
30416
108039
314941
2633299
Statistical table of small RNA classification (Total)
